Posters

This study compares a 26-color and 13-color spectral flow cytometry panel to assess the impact of reducing markers. Using PBMCs from three donors, both panels showed strong precision and high agreement (within 20%) for key immune populations. The 13-color panel maintained comparable performance, supporting its use in clinical settings when full panel complexity isn’t needed.

This study assessed three whole blood preservation methods—CryoStor CS10, Cytodelics, and Smart Tube—as PBMC alternatives for flow cytometry. Using a 26-color panel, CryoStor CS10 showed the most consistent results. Cytodelics and Smart Tube had more variability, likely due to fixatives. CryoStor is a strong alternative, though donor variability and antibody re-titration should be considered.

This study validated a qPCR assay for detecting AAV vector shedding in five body fluids. Results showed strong sensitivity and accuracy, with semen needing dilution. Variability in results was linked to pH changes in a key reagent, highlighting the importance of reagent quality control in gene therapy trials.

Over 80 PCR validations showed that acellular samples yield better precision and lower LoDs. easyMAG excelled with acellular matrices, QIAgen with cellular, and KingFisher delivered the lowest LoDs. Proper matrix-platform pairing is essential for accurate PCR bioanalysis.

Check out this poster with details on how scientists at Eurofins Clinical Trial Solutions evaluated multiple automated extraction platforms, using serum, neat urine, plasma, and peripheral blood monocuclear cells (PBMCs).

In this poster, we share describe details on how a novel electrochemiluminescence (ECL) immunoassay method was developed to overcome the technique hurdles and successfully validated for quantitatively measuring C3a/C3a desArg in human plasma samples.

Check out this poster presented at WRIB 2023 with details on how Eurofins Viracor BioPharma designed, developed, and validated assays to detect RCR and RCL vectors integrated into therapeutic T cells.