A ligand binding assay (LBA) is a highly sensitive and specific method used to detect and quantify biomolecules like proteins, peptides, or antibodies by measuring their interaction with a target ligand. LBAs are widely used in clinical trials, especially for biologics and complex therapeutics, due to their ability to detect low-abundance analytes in complex biological samples. They are versatile tools applicable to pharmacokinetics, pharmacodynamics, immunogenicity testing, and biomarker analysis, and they scale well for high-throughput screening while meeting global regulatory standards.