Comparative Performance of Bio-Rad QX200 and VibroJet DQ24 ddPCR Platforms for BRAF V600E Detection

This study compared the Bio-Rad QX200 and VibroJet DQ24 droplet digital PCR (ddPCR) systems for detecting the BRAF V600E mutation across a range of variant allele frequencies (VAFs). Using synthetic DNA samples, both systems showed strong correlation in quantitative detection, though the Bio-Rad QX200 demonstrated slightly higher precision. The VibroJet DQ24 system, however, exhibited superior qualitative sensitivity—detecting lower VAFs than the QX200—and advantages in automation, speed, and multiplexing potential. Overall, the DQ24 offers promising performance for lower VAF detection and higher throughput, while the QX200 remains strong for quantitative accuracy and established workflows.