The Importance of Pairing a Matrix with a Nucleic Acid Extraction Approach: Compilation of 66+ Bioanalytical PCR (qPCR and dPCR) Validations

This study analyzed over 80 PCR validations—66 with extraction data—across 11 biological matrices using three platforms (easyMAG, KingFisher, QIAgen). It assessed precision and limit of detection (LoD) for qPCR, RT-qPCR, and ddPCR.

  • Acellular matrices (e.g., plasma, urine) showed better precision and lower LoDs than cellular matrices.

  • easyMAG performed best with acellular samples; QIAgen was most effective with cellular ones.

  • KingFisher achieved the lowest average LoDs due to higher nucleic acid input.

  • ddPCR in PBMCs showed improved precision, though LoD varied.

Conclusion:
Pairing the right matrix with the appropriate extraction platform is crucial for reliable PCR-based bioanalysis in drug development.

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