Analytical Validation of Sanger Sequencing for the Respiratory Syncytial Virus NS2 Gene from Nasal Swabs

Title: Analytical Validation of Sanger Sequencing for the Respiratory Syncytial Virus NS2 Gene from Nasal Swabs

Nonstructural Protein II (NS2) gene sequencing assay was developed for precise analysis of respiratory syncytial virus (RSV) infection from nasal swabs. This assay demonstrates robustness and utility in both research and clinical environments, producing consistent and reliable sequencing outcomes for the NS2 gene. Sequencing allows for the monitoring of mutations, such as insertions and deletions, which could inhibit the development of effective therapies. Utilizing Sanger Sequencing, the assay offers timely and cost-effective sequencing, crucial for understanding NS2’s role in RSV infection.