PCR

We offer Quantitative PCR (qPCR) services for our clients, powered by the KingFisher Flex for automated nucleic acid extraction and the QuantStudio 7 Flex for high-precision qPCR analysis.

qPCR is a powerful technique used to amplify and quantify DNA or RNA sequences for a wide range of molecular biology applications. This service allows us to offer a streamlined, end-to-end workflow providing fast, accurate, and reproducible results, compliant with GLP, GCLP and CLIA. We employ the unidirectional flow with dedicated space for RNA/DNA extraction, master mix and amplification.

The KingFisher Flex automates the extraction of high-quality DNA and RNA from various biological sample types, ensuring consistency and minimizing manual errors which is crucial for downstream applications. The KingFisher Flex can process up to 96 samples per run, enabling rapid and scalable sample preparation and nucleic acid extraction.

Following nucleic acid extraction, the QuantStudio 7 Flex system takes over for the qPCR analysis for a wide range of applications, including gene expression studies, single nucleotide polymorphisms (SNP) genotyping, and pathogen detection, biodistribution, viral shedding among others. The system's versatility and precision support both simple and complex assays, including multiplexing for simultaneous analysis of multiple targets in a single reaction. It also accommodates 96- and 384-well plates, offering scalability for various project sizes.

Our qPCR service provides comprehensive support, from sample preparation through data analysis, ensuring a seamless and efficient process. Rigorous quality control measures are employed at every stage to ensure the highest level of accuracy and reliability in the results, adhering to GLP, GCLP and CLIA guidelines.

By leveraging the automation of KingFisher Flex and the advanced capabilities of QuantStudio 7 Flex, we can offer fast turnaround times, scalable solutions, and cost-effective services for molecular biology needs in research, pharmaceutical and biotechnology industrial applications.